Human Muscle Cell Line. These cells assume a proliferative motile phenotype when cultured in. However because immortalized cell lines often are genetically abnormal and have been maintained under artificial conditions in culture for very long periods of time which can cause them to deviate from normal function a potentially more predictive screening strategy would use primary human muscle cells.
HTERTcdk4 immortalized myogenic human cell lines represent an important tool for skeletal muscle research being used as therapeutically pertinent models of various neuromuscular disorders and in numerous fundamental studies of muscle cell function. Clonetics TM Human Skeletal Muscle Myoblasts HSMM are isolated from normal donors. HSCkM cells were cultured in DMEM high glucose supplemented with 10 FBS 1 penicillinstreptomycin and 5 µgml basic fibroblast growth factor bFGF.
TMEM16E is also expressed in somites and in developing skeletal and cardiac muscle and it is upregulated during the skeletal muscle cell line C2Cl2 myogenic differentiation.
ATCC human cells are invaluable for your public health research. Musculus pectoralis major from adult single donors lot specific source information is available on request. All cells test negative for mycoplasma bacteria yeast and fungi. Selected clones of DM1 and DM2 immortalized myoblasts behave as parental primary myoblasts with a reduced fusion capacity of immortalized DM1 myoblasts when compared with control and DM2 cells.
